Abstract: In this paper, a monaural speech enhancement method combining deep neural network (DNN) with sparse non-negative matrix factorization (SNMF) is proposed. This method takes advantage of the sparse characteristic of speech signal in time-frequency (T-F) domain and the spectral preservation characteristic of DNN presented in speech enhancement, aiming to resolve the distortion problem introduced by low SNR situation and unvoiced components without structure characteristics in conventional non-negative matrix factorization (NMF) method. Firstly, the magnitude spectrogram matrix of noisy speech is decomposed by NMF with sparse constraint to obtain the corresponding coding matrix coefficients of speech and noise dictionary. The speech and noise dictionary are pre-trained independently. Then Wiener filtering method is used to get the separated speech and noise. DNN is employed to model the non-linear function which maps the log magnitude spectrum of the separated speech from Wiener filter to the target clean speech. Evaluations are conducted on the IEEE dataset, both stationary and non-stationary types of noise are selected to demonstrate the effectiveness of the proposed method. The experimental results show that the proposed method could effectively suppress the noise and preserve the speech component from the corrupted speech signal. It has better performance than the baseline methods in terms of perceptual quality and log-spectral distortion.